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Publication: Proteolytic bacteria isolated from agro-waste dumpsites produced keratinolytic enzymes
Nnolim, N.E. Okoh, A.I. Nwodo, U.U.
Nnolim, N.E.
Okoh, A.I.
Nwodo, U.U.
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Abstract
Microbial bioconversion of carbonoclastic materials is an efficient tool for the exploitation and valorization of underutilized agro-industrial wastes. The agro-industrial sector accumulates tones of keratinous wastes biomass which may be valorized into high value products.
Consequently, the keratinolytic potentials of some bacteria isolated from terrestrial milieu was evaluated. Soil samples were collected from dumpsites, keratinase producing bacteria were isolated. Bacterial species were identified through 16S rRNA gene sequences. The keratinase activity was assessed in relation to thiol formation, percentage feather degradation and quantitation of keratinase produced. Keratinolytic bacteria were identified as Bacillus spp. (accession numbers: MG214989 - MG214992, MG214997, MG214998, MG215000, MG215002-MG215005) and Arthrobacter sp. (accession numbers; MG215001). The degree of chicken feather degradation ranged from 61.5 ± 0.71 % to 85.0 ± 1.41 %. Similarly, the activity of keratinase, total protein and thiol group ranged from 198.18 ± 15.43-731.83 ± 14.14 U/mL; 0.09 ± 0.01-0.87 ± 0.05 mg/mL; and 0.69 ± 0.12-2.89 ± 0.11 mM respectively. Notably, Bacillus sp. Nnolim-K1 displayed the best keratinolytic potential with extracellular keratinase activity and feather degradation of 731.83 ± 14.14 U/mL and 85.0 ± 1.41 % respectively, and that is an indication of a potential relevance biotechnologically.
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Date
2020-05-28
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Elsevier
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Keywords
Chicken feather,Keratinase,Thiol group,Valorisation
Citation
Nnolim NE, Okoh AI, Nwodo UU. Proteolytic bacteria isolated from agro-waste dumpsites produced keratinolytic enzymes. Biotechnol Rep (Amst). 2020 May 29;27:e00483. doi:10.1016/j.btre.2020.e00483.