Publication:
Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis patient bioaerosols

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dc.contributor.authorDinkele, R.
dc.contributor.authorGessner, S.
dc.contributor.authorMcKerry, A.
dc.contributor.authorLeonard, B.
dc.contributor.authorSeldon, R.
dc.contributor.authorKoch, A.S.
dc.contributor.authorMorrow, C.
dc.contributor.authorGqada, M.
dc.contributor.authorKamariza, M.
dc.contributor.authorBertozzi, C.R.
dc.contributor.authorSmith, B.
dc.contributor.authorMcLoud, C.
dc.contributor.authorKamholz, A.
dc.contributor.authorBryden, W.
dc.contributor.authorCall, C.
dc.contributor.authorKaplan, G.
dc.contributor.authorMizrahi, V.
dc.contributor.authorWood, R.
dc.contributor.authorWarner, D.F.
dc.contributor.departmentRyan Dinkele, Sophia Gessner, Anastasia S Koch: SAMRC/NHLS/UCT Molecular Mycobacteriology Research Uniten_US
dc.date.accessioned2024-06-07T17:29:50Z
dc.date.available2024-06-07T17:29:50Z
dc.date.epub2020-02-01
dc.date.issued2021-02-01
dc.description.abstractInterrupting transmission is an attractive anti-tuberculosis (TB) strategy but it remains underexplored owing to our poor understanding of the events surrounding transfer of Mycobacterium tuberculosis (Mtb) between hosts. Determining when live, infectious Mtb bacilli are released and by whom has proven especially challenging. Consequently, transmission chains are inferred only retrospectively, when new cases are diagnosed. This process, which relies on molecular analyses of Mtb isolates for epidemiological fingerprinting, is confounded by the prolonged infectious period of TB and the potential for transmission from transient exposures. We developed a Respiratory Aerosol Sampling Chamber (RASC) equipped with high-efficiency filtration and sampling technologies for liquid-capture of all particulate matter (including Mtb) released during respiration and non-induced cough. Combining the mycobacterial cell wall probe, DMN-trehalose, with fluorescence microscopy of RASC-captured bioaerosols, we detected and quantified putative live Mtb bacilli in bioaerosol samples arrayed in nanowell devices. The RASC enabled non-invasive capture and isolation of viable Mtb from bioaerosol within 24 hours of collection. A median 14 live Mtb bacilli (range 0-36) were isolated in single-cell format from 90% of confirmed TB patients following 60 minutes bioaerosol sampling. This represented a significant increase over previous estimates of transmission potential, implying that many more organisms might be released daily than commonly assumed. Moreover, variations in DMN-trehalose incorporation profiles suggested metabolic heterogeneity in aerosolized Mtb. Finally, preliminary analyses indicated the capacity for serial image capture and analysis of nanowell-arrayed bacilli for periods extending into weeks. These observations support the application of this technology to longstanding questions in TB transmission including the propensity for asymptomatic transmission, the impact of TB treatment on Mtb bioaerosol release, and the physiological state of aerosolized bacilli.en_US
dc.description.sponsorshipThe authors acknowledge the financial support of the South African Medical Research Council (www.samrc.ac.za) with funds from National Treasury under its Economic Competitiveness and Support Package (MRC-RFAUFSP-01-2013/CCAMP, R.W.), for ExtramuralUnit funding(to V.M.),and via the Strategic Health Innovations PartnershipsUnit (www.samrc.ac.za/ innovation/strategic-health-innovationpartnerships) (to D.F.W and V.M).en_US
dc.identifier.citationDinkele R, Gessner S, McKerry A, Leonard B, Seldon R, Koch AS, Morrow C, Gqada M, Kamariza M, Bertozzi CR, Smith B, McLoud C, Kamholz A, Bryden W, Call C, Kaplan G, Mizrahi V, Wood R, Warner DF. Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis patient bioaerosols. PLoS Pathog. 2021 Feb 1;17(2):e1009262. doi: 10.1371/journal.ppat.1009262.
dc.identifier.journalPLoS Pathogensen_US
dc.identifier.urihttps://doi.org/10.1371/journal.ppat.1009262
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/33524021/
dc.identifier.urihttps://hdl.handle.net/11288/596227
dc.language.isoenen_US
dc.publisherPLoS Oneen_US
dc.research.unitMolecular Mycobacteriologyen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectMycobacterium tuberculosis
dc.subjectBioaerosols
dc.subjectSDG-03 Good health and well-being
dc.titleCapture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis patient bioaerosolsen_US
dc.typeArticleen_US
dspace.entity.typePublication
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