Publication:
Cellular and molecular targets of waterbuck repellent blend odors in antennae of glossina fuscipes fuscipes Newstead, 1910

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dc.contributor.authorDiallo, S.
dc.contributor.authorShahbaaz, M.
dc.contributor.authorTorto, B.
dc.contributor.authorChristoffels, A.
dc.contributor.authorMasiga, D.
dc.contributor.authorGetahun, N.M.
dc.contributor.departmentSouleymane Diallo, Mohd Shahbaaz, Alan Christoffels: South African Medical Research Council Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, Cape Town, South Africaen_US
dc.date.accessioned2024-03-21T15:38:50Z
dc.date.available2024-03-21T15:38:50Z
dc.date.epub2020
dc.date.issued2020-06-03
dc.description.abstractInsects that transmit many of the world's deadliest animal diseases, for instance trypanosomosis, find their suitable hosts and avoid non-preferred hosts mostly through olfactory cues. The waterbuck repellent blend (WRB) comprising geranylacetone, guaiacol, pentanoic acid, and δ-octalactone derived from waterbuck skin odor is a repellent to some savannah-adapted tsetse flies and reduces trap catches of riverine species. However, the cellular and molecular mechanisms associated with detection and coding of the repellent odors remain to be elucidated. Here, we demonstrated that WRB inhibited blood feeding in both Glossina pallidipes Austen, 1903 and Glossina fuscipes fuscipes Newstead, 1910. Using the DREAM (Deorphanization of Receptors based on Expression Alterations in odorant receptor mRNA levels) technique, combined with ortholog comparison and molecular docking, we predicted the putative odorant receptors (ORs) for the WRB in G. f. fuscipes, a non-model insect. We show that exposure of G. f. fuscipes in vivo to WRB odorant resulted in up- and downregulation of mRNA transcript of several ORs. The WRB component with strong feeding inhibition altered mRNA transcript differently as compared to an attractant odor, showing these two odors of opposing valence already segregate at the cellular and molecular levels. Furthermore, molecular dynamics simulations demonstrated that the predicted ligand-OR binding pockets consisted mostly of hydrophobic residues with a few hydrogen bonds but a stable interaction. Finally, our electrophysiological response showed the olfactory sensory neurons of G. f. fuscipes tuned to the tsetse repellent components in different sensitivity and selectivity.en_US
dc.identifier.citationDiallo S, Shahbaaz M, Torto B, Christoffels A, Masiga D, Getahun MN. Cellular and Molecular Targets of Waterbuck Repellent Blend Odors in Antennae of Glossina fuscipes fuscipes Newstead, 1910. Front Cell Neurosci. 2020 Jun 3;14:137. doi: 10.3389/fncel.2020.00137.en_US
dc.identifier.doihttps://doi.org/10.3389/fncel.2020.00137
dc.identifier.journalOriginal Reseachen_US
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/32581714/
dc.identifier.urihttps://doi.org/10.3389/fncel.2020.00137
dc.identifier.urihttps://hdl.handle.net/11288/595583
dc.language.isoenen_US
dc.publisherFrontiers in Cellular Neuroscienceen_US
dc.research.unitBioinformatics Capacity Developmenten_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBehavior
dc.subjectMolecular docking
dc.subjectMolecular dynamics
dc.subjectOlfaction; physiology
dc.subjectRepellents
dc.subjectTsetse
dc.subjectSDG-03 Good health and well-being
dc.titleCellular and molecular targets of waterbuck repellent blend odors in antennae of glossina fuscipes fuscipes Newstead, 1910en_US
dc.typeArticleen_US
dspace.entity.typePublication
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