Loading...
GC-MS quantification of urinary symmetric dimethylarginine (SDMA), a whole-body symmetric l-arginine methylation index
Bollenbach, A Hanff, E Beckmann, B Kruger, R Tsikas, D
Bollenbach, A
Hanff, E
Beckmann, B
Kruger, R
Tsikas, D
Citations
Altmetric:
Abstract
Circulating and excretory N,N-dimethyl-l-arginine (symmetric dimethylarginine, SDMA) and N,N-dimethyl-l-arginine (asymmetric dimethylarginine, ADMA) are cardiovascular risk factors. Despite close chemical structures, the gas chromatography-mass spectrometry (GC-MS) measurement of SDMA is remarkably more difficult than that of ADMA for as yet unknown reasons. Here, we describe an improved GC-MS method for the quantitative determination of SDMA in human urine using commercially available N,N-di-[H]methyl-l-arginine (d-SDMA) as internal standard. The method is based on a single derivatization step with pentafluoropropionic anhydride (PFPA) in ethyl acetate (30 min, 65 °C) to N,N,N,O-tetrakis-pentafluoropropionyl derivatives, electron-capture negative-ion chemical ionization and selected-ion monitoring of the mass-to-charge (m/z) ions of m/z 456 for SDMA and m/z 462 for d-SDMA.
Description
Date
2018-09-01
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
ADMA,Deuterium,Guanidine methylation,Pentafluoropropionic anhydride,SDMA
Citation
Bollenbach A, Hanff E, Beckmann B, Kruger R, Tsikas D. GC-MS quantification of urinary symmetric dimethylarginine (SDMA), a whole-body symmetric l-arginine methylation index. Anal Biochem. 2018 Sep 1;556:40-44. doi: 10.1016/j.ab.2018.06.021.