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Rosmarinic acid exhibits anticancer effects via MARK4 inhibition

Anwar, S.
Shamsi, A.
Shahbaaz, M.
Queen, A.
Khan, P.
Hasan, G.M.
Islam, A.
Alajmi, M.F.
Hussain, A.
Ahmad, F.
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Abstract
Microtubule affinity regulating kinase (MARK4) is a potential drug target for different types of cancer as it controls the early step of cell division. In this study, we have screened a series of natural compounds and finally identified rosmarinic acid (RA) as a potential inhibitor of MARK4. Molecular docking and 500 ns all-atom simulation studies suggested that RA binds to the active site pocket of MARK4, forming enough number of non-covalent interactions with critical residues and MARK4-RA complex is stable throughout the simulation trajectory. RA shows an excellent binding affinity to the MARK4 with a binding constant (K) of 107 M-1. Furthermore, RA significantly inhibits MARK4 activity (IC50 = 6.204 µM). The evaluation of enthalpy change (∆H) and entropy change (∆S) suggested that the MARK4-RA complex formation is driven by hydrogen bonding and thus complexation process is seemingly specific. The consequence of MARK4 inhibition by RA was further evaluated by cell-based tau-phosphorylation studies, which suggested that RA inhibited the phosphorylation of tau. The treatment of cancer cells with RA significantly controls cell growth and subsequently induces apoptosis. Our study provides a rationale for the therapeutic evaluation of RA and RA-based inhibitors in MARK4 associated cancers and other diseases.
Description
Microtubule affinity regulating kinase (MARK4) is a potential drug target for different types of cancer as it controls the early step of cell division.
Date
2020-06-25
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Publisher
Nature Publishing Group
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Keywords
Anticancer , Drug effects , Rosmarinic acid , MARK4 protein
Citation
Anwar S, Shamsi A, Shahbaaz M, et al. Rosmarinic acid exhibits anticancer effects via MARK4 inhibition. Sci Rep 10, 10300 (2020).
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