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Inflammatory cytokine profiles of semen influence cytokine responses of cervicovaginal epithelial cells
Rametse, C.L Adefuye, A.O Olivier, A.J Curry, L Gamieldien, H Burgers, W.A Lewis, D.A Williamson, A Katz, A.A Passmore, J.S
Rametse, C.L
Adefuye, A.O
Olivier, A.J
Curry, L
Gamieldien, H
Burgers, W.A
Lewis, D.A
Williamson, A
Katz, A.A
Passmore, J.S
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Abstract
Genital inflammatory cytokine responses increase HIV risk. Since male partner semen is a complex mixture of immune-modulatory prostaglandins and cytokines, we hypothesized that exposure to semen may influence genital inflammation in women. Here, we investigated cytokine response kinetics of cervical cells following stimulation with seminal plasma from HIV-negative and HIV-positive men characterized as having low or high concentrations of inflammatory cytokines. Irrespective of the HIV status or semen cytokine profile, stimulation of cervical cells with seminal plasma resulted in significantly elevated concentrations of secreted IL-6, IL-8, TNF-β, MCP-1, GM-CSF, and VEGF within 8 h of stimulation, which tended to decline by 24 h, although this was only significant for TNF-β. Consistent with this, cervical cells responded to seminal plasma with increases in IL-8 and IL-1β mRNA expression of 10-fold. These findings suggest that the impact of semen on local female genital cytokines is likely transient. Although these findings suggest that the impact of semen on local female genital cytokines may not be sustained long-term, this heightened genital inflammation may have implications for HIV risk in women.
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Date
2018-12-04
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Frontiers
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Keywords
HIV,HeLa,cytokines,genital inflammation,interleukins,semen
Citation
Rametse CL, Adefuye AO, Olivier AJ, Curry L, Gamieldien H, Burgers WA, Lewis DA, Williamson AL, Katz AA, Passmore JS. Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells. Front Immunol. 2018 Dec 4;9:2721. doi: 10.3389/fimmu.2018.02721.