Loading...
Molecular identification of some wild Nigerian mushrooms using internal transcribed spacer: Polymerase chain reaction
Adeniyi, M ; Titilawo, Y ; Oluduro, A ; Odeyemi, O ; Nakin, M ; Okoh, AI
Adeniyi, M
Titilawo, Y
Oluduro, A
Odeyemi, O
Nakin, M
Okoh, AI
Citations
Altmetric:
Abstract
Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)-polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected at Environmental Pollution Science and Technology farm, Ilesa, Southwest Nigeria. The PCR amplification of ITS1 and 4 of the mushrooms isolates yielded approximately 850 bp. Amplicons obtained were sequenced and identified using BLASTn in the NCBI. The BLASTn results revealed that Termitomyces aurantiacus (3), Tricholoma matsutake (8), Tricholoma robustum (2), P. ostreatus (4), Schizophyllum commune (1) and Pleurotus pulmonarius (1) were fully represented. Only Tricholoma matsutake (KT273371), Pleurotus pulmonarius (KY962469) and Tricholoma matsutake (AF438605) had 100% similarity with reference strain. However, the phylogenetic analysis of the isolates showed low genetic relatedness with reference strains. This study revealed the novelty of the mushroom strains and thus advocating the need for strict conservation measures and further investigations on their potential benefits to mankind.
Description
Date
2018-09-20
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Nature
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
ENPOST,Food security,Molecular identification,Mushrooms,Nigeria
Citation
Adeniyi M, Titilawo Y, Oluduro A, Odeyemi O, Nakin M, Okoh AI. Molecular identification of some wild Nigerian mushrooms using internal transcribed spacer: polymerase chain reaction. AMB Express. 2018;8(1):148. DOI: 10.1186/s13568-018-0661-9.