Bollenbach, AHanff, EBeckmann, BKruger, RTsikas, D2024-10-022024-10-022018-09-01Bollenbach A, Hanff E, Beckmann B, Kruger R, Tsikas D. GC-MS quantification of urinary symmetric dimethylarginine (SDMA), a whole-body symmetric l-arginine methylation index. Anal Biochem. 2018 Sep 1;556:40-44. doi: 10.1016/j.ab.2018.06.021.https://pubmed.ncbi.nlm.nih.gov/29944873/https://doi.org/10.1016/j.ab.2018.06.021https://hdl.handle.net/11288/597675Circulating and excretory N,N-dimethyl-l-arginine (symmetric dimethylarginine, SDMA) and N,N-dimethyl-l-arginine (asymmetric dimethylarginine, ADMA) are cardiovascular risk factors. Despite close chemical structures, the gas chromatography-mass spectrometry (GC-MS) measurement of SDMA is remarkably more difficult than that of ADMA for as yet unknown reasons. Here, we describe an improved GC-MS method for the quantitative determination of SDMA in human urine using commercially available N,N-di-[H]methyl-l-arginine (d-SDMA) as internal standard. The method is based on a single derivatization step with pentafluoropropionic anhydride (PFPA) in ethyl acetate (30 min, 65 °C) to N,N,N,O-tetrakis-pentafluoropropionyl derivatives, electron-capture negative-ion chemical ionization and selected-ion monitoring of the mass-to-charge (m/z) ions of m/z 456 for SDMA and m/z 462 for d-SDMA.enADMADeuteriumGuanidine methylationPentafluoropropionic anhydrideSDMAArticle