Nunes, M.CWeinberg, ACutland, C.LJones, SWang, DDighero-Kemp, BLevine, M.ZWairagkar, NMadhi, S.A2024-12-292024-12-292018-12-31Nunes MC, Weinberg A, Cutland CL, Jones S, Wang D, Dighero-Kemp B, Levine MZ, Wairagkar N, Madhi SA. Neutralization and hemagglutination-inhibition antibodies following influenza vaccination of HIV-infected and HIV-uninfected pregnant women. PLoS One. 2018 Dec 31;13(12):e0210124. doi: 10.1371/journal.pone.0210124.10.1371/journal.pone.0210124https://pubmed.ncbi.nlm.nih.gov/30596775/https://doi.org/10.1371/journal.pone.0210124https://hdl.handle.net/11288/597951Background: We previously reported that despite HIV-infected pregnant women had modest humoral immune responses to inactivated influenza vaccine (IIV) measured by hemagglutination-inhibition (HAI) assay, the observed vaccine efficacy against influenza disease was higher than predicted by HAI; suggesting that IIV may confer protection to HIV-infected individuals by additional mechanisms. We evaluated the response to IIV by microneutralization (MN) and HAI assays and correlated both methods in HIV-infected and HIV-uninfected pregnant women. Methods: MN and HAI antibodies were measured pre-vaccination and approximately one-month post-vaccination in 80 HIV-infected and 75 HIV-uninfected women who received IIV. Geometric mean titers (GMTs), fold-change in titers and seroconversion rates were determined for the three influenza stains in the vaccine. Results: After vaccination there were significant increases in MN and HAI GMTs for the three vaccine strains in both HIV-infected and HIV-uninfected women. HIV-infected women had, however, a lower immune response compared to HIV-uninfected. Fold-increases were 2 to 3-times higher for MN assay compared to HAI assay for the influenza-A strains. Also a higher percentage of women seroconverted by MN than by HAI assay for the influenza-A strains. There was high positive correlation between MN and HAI assays, except for the B/Victoria strain at pre-vaccination. Conclusions: In general, the MN assay was more sensitive than the HAI assay. Microneutralization antibodies might correlate better with protection against influenza infection.enhttp://creativecommons.org/licenses/by/4.0/article1932-6203